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10x Buffer Ecl136II, PacI, SacI


Optimal buffer recommended for use with Ecl136II, PacI, SacI restriction enzymes. Premixed with BSA for enhanced stability.
  
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To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

The following enzymes require unique buffers for optimal digestion: 

  
Storage Condition-20 C
HazardousNo
1X Composition
  • 10 mM Bis-Tris Propane-HCl (pH 6.5 at 37°C)
  • 10 mM MgCl2 
  • 0.1 mg/mL BSA