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Home | Molecular Biology | Restriction & Modifying Enzymes | Restriction Enzymes | Buffers & Reagents

10x Buffer EcoRI


Optimal buffer recommended for use with EcoRI restriction enzyme. Premixed with BSA for enhanced stability.
  
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To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

The following enzymes require unique buffers for optimal digestion: 

  
Storage Condition-20 C
HazardousNo
1X Composition
  • 50 mM Tris-HCl (pH 7.5 at 37°C)
  • 10 mM MgCl2 
  • 100 mM NaCl
  • 0.02% Triton X-100
  • 0.1 mg/mL BSA

Protocols and Product Manuals

pdf   10x Buffer EcoRI - Product Information