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AanI (PsiI)

5'...T T A↓T A A...3'
3'...A A T↑A T T...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available Optimal incubation at 37°C Tango buffer for 100% activity Thermal inactivation at 65°C in 20 min Genome qualified LO certified

The AanI (PsiI) restriction enzyme recognizes TTA^TAA sites and cuts best at 37°C in Tango buffer (Isoschizomers: PsiI)
  
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Lambda DNA digested with AanI (PsiI)

Lambda DNA digested with AanI (PsiI), 0.7% agarose, 12 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Conditions for 100% Activity
  • 1X Buffer Tango: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA
  • Incubate at 37°C
Digestion of Agarose-embedded DNAMinimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
Storage BufferAanI is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango
(yellow)
1X / 2X
AanI Buffer (Unique) 37°C 50-100 50-100 0-20 0-20 100 20-50 1X or 2X

Methylation Effects

  • Dam: never overlaps - no effect.
  • Dcm: never overlaps - no effect.
  • CpG: never overlaps - no effect.
  • EcoKI: may overlap - effect not determined.
  • EcoBI: never overlaps - no effect.

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
0% 0-20% 20-50% 50-100%

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
12 1 2 0 0 0
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
1 1 1 1 1 0

New sites generated by ligation

Newly generated recognition sites resulting from ligation of blunt DNA ends

Recognition sequence Second restriction enzyme Restriction enzymes cleaving the newly generated recognition sequence
TTA^TAA
  • Bst1107I (BstZ17I)/FastDigest BstZ17I (Bst1107I) (GTA^TAC)
  • FaiI* (YA^TA)
  • FaiI* (YA^TG)
  • FaiI
  • DraI/FastDigest DraI (TTT^AAA)
  • Eco32I (EcoRV)/FastDigest EcoRV (Eco32I) (GAT^ATC)
  • HincII (HindII)/FastDigest HincII* (GTY^AAC)
  • KspAI (HpaI)/FastDigest HpaI (KspAI) (GTT^AAC)
  • MssI (PmeI)/FastDigest MssI (GTTT^AAAC)
  • RsaI/FastDigest RsaI (GT^AC)
  • ScaI/FastDigest ScaI (AGT^ACT)
  • SmiI (SwaI)/FastDigest SwaI (SmiI) (ATTT^AAAT)
  • FastDigest MseI (SaqAI)
  • Tru1I (MseI)/FastDigest Tru1I
  • Eco147I (StuI)/FastDigest StuI (Eco147I) (AGG^CCT)
  • SetI
  • Eco47III (AfeI)/FastDigest AfeI (Eco47III) (AGC^GCT)
  • AluI/FastDigest AluI
  • CviJI
  • SetI
  • EheI (SfoI)/FastDigest EheI (GGC^GCC)
  • CviJI
  • SspI/FastDigest SspI (AAT^ATT)
  • FastDigest MseI (SaqAI)
  • TasI (Tsp509I)/FastDigest Tsp509I (TasI)
  • Tru1I (MseI)/FastDigest Tru1I