Recently Viewed

Home | Molecular Biology | Restriction & Modifying Enzymes | Restriction Enzymes | Conventional

AjiI (BmgBI)

5'...C A C↓G T C...3'
3'...G T G↑C A G...5'

Unique buffer for 100% activity Optimal incubation at 37°C Cleavage blocked or impaired by CpG methylation Thermal inactivation at 65°C in 20 min Genome qualified LO certified

The AjiI (BmgBI) restriction enzyme recognizes CAC^GTC sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BmgBI, BtrI)
  
Loading...
Lambda DNA digested with AjiI

Lambda DNA digested with AjiI, 0.7% agarose, 17 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Conditions for 100% Activity
  • 1X Buffer AjiI: 10 mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10 mM MgCl2, 100 mM KCl and 0.1 mg/mL BSA
  • Incubate at 37°C
Digestion of Agarose-embedded DNAMinimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
NoteLow salt, high glycerol ( > 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango (yellow)
1X / 2X
AjiI (unique) 37°C NR NR 20-50† NR NR 20-50† 2X†

† Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Methylation Effects

  • Dam: never overlaps - no effect
  • Dcm: never overlaps - no effect
  • CpG: completely overlaps - blocked
  • EcoKI: may overlap - effect not determined
  • EcoBI: may overlap - effect not determined
Methylation type Sequence Cleavage effect
CpGCACGTC Blocked

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
50 to 100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
17 0 0 0 0 0
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
0 0 0 0 0 0

New sites generated by ligation

Newly generated recognition sites resulting from ligation of blunt DNA ends

Recognition sequence Second restriction enzyme Restriction enzymes cleaving the newly generated recognition sequence
CAC^GTC
  • AluI/FastDigest AluI (AG^CT)
  • CviJI* (RG^CT)
  • MspA1I* (CMG^CTG)
  • PvuII/FastDigest PvuII (CAG^CTG)
  • SetI
  • Ecl136II (EcoICRI)/FastDigest Ecl136II (GAG^CTC)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (CCG^CTC)
  • MnlI/FastDigest MnlI
  • SetI
  • Eco105I (SnaBI)/FastDigest SnaBI (Eco105I) (TAC^GTA)
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)* (YAC^GTA)
  • MaeII
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)
  • SetI
  • TaiI (MaeII)/FastDigest TaiI
  • Eco72I (PmlI)/FastDigest PmlI (Eco72I) (CAC^GTG)
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)* (YAC^GTG)
  • Eco72I (PmlI)/FastDigest PmlI (Eco72I)
  • MaeII
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)
  • SetI
  • TaiI (MaeII)/FastDigest TaiI
  • FaiI* (YA^TG)
  • TscAI (TspRI)/FastDigest TspRI (TscAI)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (GAG^CGG)
  • MspA1I* (CMG^CGG)
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • PdiI (NaeI)/FastDigest NaeI (PdiI) (GCC^GGC)
  • BceAI
  • ZraI (GAC^GTC)
  • AjiI (BmgBI)
  • MaeII
  • SetI
  • TaiI (MaeII)/FastDigest TaiI
GAC^GTG
  • AluI/FastDigest AluI (AG^CT)
  • CviJI* (RG^CT)
  • MspA1I* (CMG^CTG)
  • PvuII/FastDigest PvuII (CAG^CTG)
  • SetI
  • DpnI/FastDigest DpnI (GA^TC)
  • HinfI/FastDigest HinfI
  • Ecl136II (EcoICRI)/FastDigest Ecl136II (GAG^CTC)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (CCG^CTC)
  • MnlI/FastDigest MnlI
  • SetI
  • Eco105I (SnaBI)/FastDigest SnaBI (Eco105I) (TAC^GTA)
  • Eco72I (PmlI)/FastDigest PmlI (Eco72I) (CAC^GTG)
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)* (YAC^GTA)
  • Ppu21I (BsaAI)/FastDigest BsaAI (Ppu21I)* (YAC^GTG)
  • MaeII
  • SetI
  • TaiI (MaeII)/FastDigest TaiI
  • Eco47III (AfeI)/FastDigest AfeI (Eco47III) (AGC^GCT)
  • FspAI/FastDigest FspAI* (RTGC^GCAC)
  • FspAI/FastDigest FspAI* (RTGC^GCAT)
  • NsbI (FspI)/FastDigest FspI (NsbI) (TGC^GCA)
  • CseI (HgaI)/FastDigest HgaI (CseI)
  • EheI (SfoI)/FastDigest EheI (GGC^GCC)
  • CseI (HgaI)/FastDigest HgaI (CseI)
  • Hin1I (BsaHI)/FastDigest BsaHI (Hin1I)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (GAG^CGG)
  • MspA1I* (CMG^CGG)
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • PdiI (NaeI)/FastDigest NaeI (PdiI) (GCC^GGC)
  • BceAI
  • ZraI (GAC^GTC)
  • AatII/FastDigest AatII
  • Hin1I (BsaHI)/FastDigest BsaHI (Hin1I)
  • MaeII
  • SetI
  • TaiI (MaeII)/FastDigest TaiI
  • ZraI