Previously added items:
5'...C↓A C G A G...3'3'...G T G C T↑C...5'
Lambda DNA 0.7% agarose, 8 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
For methylation sensitivity refer to product specifications.
1X Buffer Tango:
BauI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.15% Triton X-100, 0.2 mg/mL BSA and 50% (v/v) glycerol.
Thermo Scientific conventional restriction endonucleases are classic restriction enzymes, which normally digest DNA in one hour and each require a specific reaction buffer for 100% activity.