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Bme1390I (ScrFI)

5'...C C↓N G G...3'
3'...G G N↑C C...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available O buffer for 100% activity Optimal incubation at 37°C Cleavage blocked or impaired by overlapping Dcm methylation Cleavage blocked or impaired by overlapping CpG methylation Thermal inactivation at 80°C in 20 min LO certified

The Bme1390I (ScrFI) restriction enzyme recognizes CC^NGG sites and cuts best at 37°C in O buffer (Isoschizomers: BmrFI, BssKI, BstSCI, MspR9I, ScrFI, StyD4I)
  
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Lambda DNA (dcm-) digested with Bme1390I

Lambda DNA (dcm-) 1.4% agarose, 185 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

Assayed using lambda DNA (dcm-) (#SD0021). Bme1390I is blocked by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Compatible EndsCC↓SGG - BcnI, SatI
CC↓WGG - MvaI, SatI
Conditions for 100% Activity
  • 1X Buffer O: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl, and 0.1 mg/ml BSA
  • Incubate at 37°C
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
Storage BufferBme1390I is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango (yellow)
1X / 2X
O (Orange) 37°C 20-50 50-100 100 50-100 50-100 50-100 1X or 2X

Incubation at 30°C results in a 2.5-fold increase in activity.

Methylation Effects

Methylation type Sequence Cleavage effect
Dcm (CCWGG) 5'...Cm5CW GG...3'
3'...G GWm5CC...5'		 Blocked
CpG 5'...Cm5C GGG...3'
3'...G Gm5CCC...5'		 Blocked

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
20-50 50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
185 3 11 16 12 12
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
10 10 11 11 14 22

New sites generated by ligation

Newly generated recognition sites resulting from fill-in of 5'-overhang and self-ligation

Recognition Sequence Newly generated sequence after reaction Enzymes that cleave the newly generated sequence
CC^NGGCCNNGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
CC^AGGCCAAGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • Eco130I (StyI)/FastDigest StyI (Eco130I)
CC^CGGCCCCGG
  • [BcnI (NciI)/FastDigest NciI (BcnI)]
  • [Bme1390I (ScrFI)/FastDigest ScrFI (Bme1390I)]
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • [BssKI]
  • [HpaII/FastDigest HpaII]
  • [MspI (HpaII)/FastDigest MspI]
CC^GGGCCGGGG
  • [BcnI (NciI)/FastDigest NciI (BcnI)]
  • [Bme1390I (ScrFI)/FastDigest ScrFI (Bme1390I)]
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • [BssKI]
  • [HpaII/FastDigest HpaII]
  • [MspI (HpaII)/FastDigest MspI]
CC^TGGCCTTGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • Eco130I (StyI)/FastDigest StyI (Eco130I)