Previously added items:
5'...C C↓T N A G C...3'3'...G G A N T↑C G...5'
Lambda DNA digested with Bpu10I, 0.7% agarose, 19 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
A large excess of enzyme (> 4 U/1 µg DNA) may result in incomplete DNA cleavage. Therefore, we recommend increasing the incubation time instead of using an excess of Bpu10I. Low salt, high glycerol (> 5%) concentrations, pH > 7.0, or a large excess of enzyme may result in star activity. For cleavage with Bpu10I, at least two copies of its recognition sequence are required.
For methylation sensitivity refer to product specifications.
† Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).