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BseSI (Bme1580I)

5'...G K G C M↓C...3'
3'...C↑M C G K G...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available G buffer for 100% activity Optimal incubation at 55°C Only small amounts (up to 10 u) can be inactivated at 80°C in 20 min Genome qualified LO certified

The BseSI (Bme1580I) restriction enzyme recognizes GKGCM^C sites and cuts best at 55°C in G buffer (Isoschizomers: BaeGI, BstSLI)
  
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Lambda DNA digested with BseSI (Bme1580I)

Lambda DNA digested with BseSI (Bme1580I), 0.7% agarose, 10 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Compatible EndsGGGCC↓C - ApaI, Eco24I, SduI
GTGCA↓C - Alw21I, Mph1103I, PstI, SdaI, SduI
Conditions for 100% Activity
  • 1X Buffer G: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl, and 0.1 mg/mL BSA
  • Incubate at 55°C
Digestion of Agarose-embedded DNAMinimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
NoteIncubation at 37°C results in 20% activity. Low salt, high glycerol (> 5%) concentrations, pH  > 8.0, or a large excess of enzyme may result in star activity.
Storage BufferBseSI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango (yellow)
1X / 2X
G 55°C 20-50 100 0-20 20-50 50-100 0-20 1X

Methylation Effects

Methylation type Sequence Cleavage effect
Dcm (CCWGG) 5'...GKGCCm5CW GG...3'
3'...CMCGG GWm5CC...5'		 No effect
CpG 5'...m5C GKGCMm5C G...3'
3'... Gm5CMCGK Gm5C...5'		 No effect
EcoKI (AAC(N)6GTGC) 5'...Am6AC(N)6G TGCMC...3'
3'...T TG(N)6Cm6ACGKG...5'		 Impaired

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
10 1 2 3 3 4
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
2 3 3 3 2 1

New sites generated by ligation

Newly generated recognition sites resulting from ligation of protruding compatible DNA ends

Recognition Sequence Second Enzyme Enzymes recognizing newly generated recognition sequence
GGGCA^C
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)* (GGGCA^C)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GGGCC^C
  • ApaI/FastDigest ApaI (GGGCC^C)
  • Eco24I (BanII)* (GGGCC^C)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)* (GGGCC^C)
  • ApaI/FastDigest ApaI
  • BmgT120I
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • Bsp120I (PspOMI)/FastDigest Bsp120I
  • BspLI (NlaIV)/FastDigest NlaIV (BspLI)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • Cfr13I (Sau96I)/FastDigest Sau96I (Cfr13I)
  • CviJI
  • Eco24I (BanII)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GTGCA^C
  • Alw21I (BsiHKAI)/FastDigest Alw21I* (GTGCA^C)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)* (GTGCA^C)
  • Alw21I (BsiHKAI)/FastDigest Alw21I
  • Alw44I (ApaLI)/FastDigest ApaLI (Alw44I)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • Hpy8I (MjaIV)/FastDigest Hpy8I
  • HpyCH4V
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
  • Mph1103I (NsiI)/FastDigest NsiI (Mph1103I) (ATGCA^T)
  • HpyCH4V
  • PstI/FastDigest PstI (CTGCA^G)
  • SdaI (SbfI)/FastDigest SbfI (SdaI) (CCTGCA^GG)
  • BsgI
  • HpyCH4V
GTGCC^C
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)* (GTGCC^C)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)