Previously added items:
5'...C C A N N N N N↓N T G G...3'3'...G G T N↑N N N N N A C C...5'
Lambda DNA digested with BstXI, 0.7% agarose, 13 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Incubation at 37°C results in 50% activity. Greater than 15-fold overdigestion with BstXI may result in star activity. BstXI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.
For methylation sensitivity refer to product specifications.
Cm5CA GGNNCm5CT GG
G GTm5CCNNG GAm5CC