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BsuRI (HaeIII)

5'...G G↓C C...3'
3'...C C↑G G...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available R buffer for 100% activity Optimal incubation at 37°C Thermal inactivation at 80°C in 20 min LO certified

The BsuRI (HaeIII) restriction enzyme recognizes GG^CC sites and cuts best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI)
  
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Lambda DNA digested with BsuRI (HaeIII)

Lambda DNA digested with BsuRI (HaeIII), 1.4% agarose, 149 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Conditions for 100% Activity
  • 1X Buffer R: 10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl, and 0.1 mg/mL BSA
  • Incubate at 37°C
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
Storage BufferBsuRI is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango
(yellow)
1X / 2X
R (Red) 37°C 0-20 0-20 0-20 100 50-100 100 1X or 2X

Methylation Effects

Methylation type Sequence Cleavage effect
Dcm (CCWGG) 5'...GGCm5CW GG...3'
3'...CCG GWm5CC...5'		 No effect
Dcm (CCWGG) 5'...Cm5CW GGCm5CW GG...3'
3'...G GWm5CCG GWm5CC...5'		 No effect
CpG 5'...m5C GGCm5C G...3'
3'... Gm5CCG Gm5C...5'		 No effect

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
0-20 20-50 50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
149 11 15 22 11 13
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
12 14 14 14 13 24

New sites generated by ligation

Newly generated recognition sites resulting from ligation of blunt DNA ends

Recognition sequence Second restriction enzyme Restriction enzymes cleaving the newly generated recognition sequence
GG^CC
  • AluI/FastDigest AluI (AG^CT)
  • CviJI* (RG^CT)
  • Ecl136II (EcoICRI)/FastDigest Ecl136II (GAG^CTC)
  • Eco47III (AfeI)/FastDigest AfeI (Eco47III) (AGC^GCT)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (CCG^CTC)
  • MspA1I* (CMG^CTG)
  • PvuII/FastDigest PvuII (CAG^CTG)
  • CviJI
  • Bst1107I (BstZ17I)/FastDigest BstZ17I (Bst1107I) (GTA^TAC)
  • Csp6I (CviQI)/FastDigest Csp6I
  • RsaI/FastDigest RsaI
  • CviJI* (RG^CC)
  • Eco147I (StuI)/FastDigest StuI (Eco147I) (AGG^CCT)
  • MlsI (MscI)/FastDigest MscI (MlsI) (TGG^CCA)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • CviJI
  • Eco32I (EcoRV)/FastDigest EcoRV (Eco32I) (GAT^ATC)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • BspPI (AlwI)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • EheI (SfoI)/FastDigest EheI (GGC^GCC)
  • BmgT120I
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • Cfr13I (Sau96I)/FastDigest Sau96I (Cfr13I)
  • CviJI
  • FspAI/FastDigest FspAI* (RTGC^GCAC)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
  • HincII (HindII)/FastDigest HincII* (GTY^GAC)
  • FaqI (BsmFI)/FastDigest BsmFI (FaqI)