Previously added items:
5'...G A C G C (N)5 ↓...3'3'...C T G C G (N)10↑...5'
pBR322 DNA digested with CseI (HgaI), 1.4% agarose, 11 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Assayed using pBR322 DNA (#SD0041). Low salt, high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity. CseI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation, or heat the digested DNA in the presence of SDS prior to electrophoresis.
For methylation sensitivity refer to product specifications.
† Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).