5'...G A C N N N↓N N G T C...3'3'...C T G N N↑N N N C A G...5'
Lambda DNA digested with Eam1105I (AhdI), 0.7% agarose, 9 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Low salt, high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity.
For methylation sensitivity refer to product specifications.
GAm5C G(N)3m5C GTC
CT Gm5C(N)3 Gm5CAG
m5C GAC(N)5GTm5C G