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Home | Molecular Biology | Restriction & Modifying Enzymes | Restriction Enzymes | Conventional

HincII (HindII)

5'...G T Y↓R A C...3'
3'...C A R↑Y T G...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available Tango buffer for 100% activity Optimal incubation at 37°C Cleavage blocked or impaired by overlapping CpG methylation Thermal inactivation at 65°C in 20 min LO certified

The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII)
  
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Lambda DNA digested with HincII (HindII)

Lambda DNA digested with HincII (HindII), 1% agarose, 35 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Conditions for 100% Activity
  • 1X Buffer Tango: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA
  • Incubate at 37°C
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
Storage BufferHincII is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango 
(yellow)
1X / 2X
Tango 37°C 50-100 50-100 20-50 50-100 100 50-100 1X or 2X

Methylation Effects

Methylation type Sequence Cleavage effect
CpG 5'...GTYRAm5C G...3'
3'...CARYT Gm5C...5'		 Impaired
CpG 5'...m5C GTYRAm5C G...3'
3'... Gm5CARYT Gm5C...5'		 Blocked
CpG 5'...GTm5C GAC...3'
3'...CA Gm5CTG...5'		 Impaired
EcoBI (TGA(N)8TGCT) 5'...GTTGm6AC(N)7 TGCT...3'
3'...CAAC TG(N)7m6ACGA...5'		 Blocked
EcoKI (AAC(N)6GTGC) 5'...GTYAm6AC(N)6G TGC...3'
3'...CART TG(N)6Cm6ACG...5'		 Blocked

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
35 13 1 2 1 1
pTZ19R/U pTZ57R pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
1 1 1 1 1 2

New sites generated by ligation

Newly generated recognition sites resulting from ligation of blunt DNA ends

Recognition sequence Second restriction enzyme Restriction enzymes cleaving the newly generated recognition sequence
GTC^RAC
  • Bsp68I (NruI)/FastDigest NruI (RruI) (TCG^CGA)
  • Hpy188I
  • Bst1107I (BstZ17I)/FastDigest BstZ17I (Bst1107I) (GTA^TAC)
  • Hpy8I (MjaIV)/FastDigest Hpy8I
  • XmiI (AccI)/FastDigest AccI (XmiI)
  • DpnI/FastDigest DpnI (GA^TC)
  • Alw26I (BsmAI)/FastDigest Alw26I
  • Ecl136II (EcoICRI)/FastDigest Ecl136II (GAG^CTC)
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (CCG^CTC)
  • MnlI/FastDigest MnlI
  • KspAI (HpaI)/FastDigest HpaI (KspAI) (GTT^AAC)
  • HincII (HindII)/FastDigest HincII
  • Hpy8I (MjaIV)/FastDigest Hpy8I
  • MbiI (BsrBI)/FastDigest BsrBI (MbiI)* (GAG^CGG)
  • MspA1I* (CMG^CGG)
  • HpaII/FastDigest HpaII
  • MspI (HpaII)/FastDigest MspI
  • RsaI/FastDigest RsaI (GT^AC)
  • ScaI/FastDigest ScaI (AGT^ACT)
  • MaeIII
  • NmuCI (Tsp45I)/FastDigest NmuCI
GTT^RAC
  • AanI (PsiI)/FastDigest PsiI (AanI) (TTA^TAA)
  • Bsp68I (NruI)/FastDigest NruI (RruI) (TCG^CGA)
  • DraI/FastDigest DraI (TTT^AAA)
  • MssI (PmeI)/FastDigest MssI (GTTT^AAAC)
  • SmiI (SwaI)/FastDigest SwaI (SmiI) (ATTT^AAAT)
  • FastDigest MseI (SaqAI)
  • Tru1I (MseI)/FastDigest Tru1I
  • Bsp68I (NruI)/FastDigest NruI (RruI) (TCG^CGA)
  • TaqI/FastDigest TaqI
  • Bst1107I (BstZ17I)/FastDigest BstZ17I (Bst1107I) (GTA^TAC)
  • Hpy8I (MjaIV)/FastDigest Hpy8I
  • FspAI/FastDigest FspAI* (RTGC^GCAC)
  • FspAI/FastDigest FspAI* (RTGC^GCAT)
  • NsbI (FspI)/FastDigest FspI (NsbI) (TGC^GCA)
  • HpyCH4V
  • KspAI (HpaI)/FastDigest HpaI (KspAI) (GTT^AAC)
  • HincII (HindII)/FastDigest HincII
  • Hpy8I (MjaIV)/FastDigest Hpy8I
  • KspAI (HpaI)/FastDigest HpaI (KspAI)
  • FastDigest MseI (SaqAI)
  • Tru1I (MseI)/FastDigest Tru1I
  • RsaI/FastDigest RsaI (GT^AC)
  • ScaI/FastDigest ScaI (AGT^ACT)
  • MaeIII