Previously added items:
5'...G C A G C (N)8 ↓...3'3'...C G T C G (N)12↑...5'
Lambda DNA digested with Lsp1109I (BbvI), 1.4% agarose, 199 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Assayed using pBR322 DNA (#SD0041). Lsp1109I may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. Greater than 32-fold overdigestion with Lsp1109I may result in star activity.
For methylation sensitivity refer to product specifications.
† Star activity appears at a greater than 5-fold overdigestion (5 units × 1 hour).
m5C GCAGm5C G