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Home | Molecular Biology | Restriction & Modifying Enzymes | Restriction Enzymes | Conventional

MauBI

5'...C G↓C G C G C G...3'
3'...G C G C G C↑G C...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available Tango buffer for 100% activity Optimal incubation at 37°C Cleavage blocked or impaired by CG methylation Genome qualified Thermal inactivation at 65°C, 20 min. LO certified

The MauBI restriction enzyme recognizes CG^CGCGCG sites and cuts best at 37°C in Tango buffer
  
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Ad2 DNA digested with MauBI

Ad2 DNA digested with MauBI, 0.7% agarose, 8 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Compatible EndsAflIII (A↓CGCGT), BtgI (C↓CGCGG), MluI, PauI, SgsI.
Conditions for 100% Activity
  • 1X Buffer Tango: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate, and 0.1 mg/mL BSA
  • Incubate at 37°C
Digestion of Agarose-embedded DNAMinimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
NoteAssayed using linearized pJET1 DNA with inserted MauBI recognition site.
Storage BufferMauBI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango
(yellow)
1X / 2X
Tango 37°C 0-20 0-20 0-20 0-20 100 0-20 1X

Methylation Effects

Methylation type Sequence Cleavage effect
CpGCGCGCGCG Blocked

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
0 0-20 20-50

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
0 0 0 0 0 0
pTZ19R/U pTZ57R pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
0 0 0 0 0 0

New sites generated by ligation

Newly generated recognition sites resulting from ligation of protruding compatible DNA ends

Recognition SequenceSecond EnzymeEnzymes recognizing newly generated recognition sequence
CG^CGCGCG
  • AflIII* (A^CGCGT)
  • BtgI* (C^CGCGG)
  • MluI/FastDigest MluI (A^CGCGT)
  • Bsh1236I (BstUI)/FastDigest Bsh1236I
  • HhaI/FastDigest HhaI
  • Hin6I (HinP1I)/FastDigest HinP1I (Hin6I)
  • PauI (BssHII)/FastDigest BssHII (PteI) (G^CGCGC)
  • SgsI (AscI)/FastDigest AscI (SgsI) (GG^CGCGCC)
  • Bsh1236I (BstUI)/FastDigest Bsh1236I
  • Cac8I
  • HhaI/FastDigest HhaI
  • Hin6I (HinP1I)/FastDigest HinP1I (Hin6I)
  • PauI (BssHII)/FastDigest BssHII (PteI)

Newly generated recognition sites resulting from fill-in of 5'-overhang and self-ligation

Recognition SequenceNewly generated sequence after reactionEnzymes that cleave the newly generated sequence
CG^CGCGCGCGCGCGCGCGCG
  • [Bsh1236I (BstUI)/FastDigest Bsh1236I]
  • [Cac8I]
  • [HhaI/FastDigest HhaI]
  • [Hin6I (HinP1I)/FastDigest HinP1I (Hin6I)]
  • [MauBI/FastDigest MauBI]
  • [PauI (BssHII)/FastDigest BssHII (PteI)]