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MvaI (BstNI)

5'...C C↓W G G...3'
3'...G G W↑C C...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available R buffer for 100% activity Optimal incubation at 37°C Not inactivated at 80°C in 20 min Recombinant enzyme LO certified

The MvaI (BstNI) restriction enzyme recognizes CC^WGG sites and cuts best at 37°C in R buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI)
  
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Lambda DNA digested with MvaI (BstNI)

Lambda DNA digested with MvaI (BstNI), 1.4% agarose, 71 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Compatible EndsSatI, Bme1390I.
Conditions for 100% Activity
  • 1X Buffer R: 10 mM Tris-HCl (pH 8.5 at 37°C), 10 mM MgCl2, 100 mM KCl, and 0.1 mg/mL BSA
  • Incubate at 37°C
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
NoteLow salt, high glycerol (>5%) concentrations or a large excess of enzyme may result in star activity. Unlike its neoschizomer EcoRII, MvaI does not require multiple copies of recognition site for efficient cleavage.
Storage BufferMvaI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 400 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango
(yellow)
1X / 2X
R (Red) 37°C 20-50 20-50 50-100 100 20-50† 100 1X† or 2X

† Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Methylation Effects

Methylation type Sequence Cleavage effect
Dcm (CCWGG)Cm6CWGG No effect

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
0 20-50 50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
71 2 7 6 5 5
pTZ19R/U pTZ57R pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
5 5 5 5 8 12

New sites generated by ligation

Newly generated recognition sites resulting from fill-in of 5'-overhang and self-ligation

Recognition Sequence Newly generated sequence after reaction Enzymes that cleave the newly generated sequence
CC^WGGCCWWGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • Eco130I (StyI)/FastDigest StyI (Eco130I)
CC^AGGCCAAGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • Eco130I (StyI)/FastDigest StyI (Eco130I)
CC^TGGCCTTGG
  • BseDI (BsaJI)/FastDigest BsaJI (BseDI)
  • Eco130I (StyI)/FastDigest StyI (Eco130I)