Previously added items:
5'...C T G C A↓G...3'3'...G↑A C G T C...5'
Lambda DNA digested with PstI, 0.7% agarose, 28 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Conditions of high pH, low salt, high glycerol, 8% DMSO can cause star activity (Malyguine, E., et al., Gene, 8, 163-177, 1980). Surrounding sequences: the presence of adjacent runs of G-C base pairs confers significant resistance to cleavage (K. Armstrong, W.R. Bauer. NAR 10, 993-1007, 1982). 100% dUTP incorporation at the recognition site reduces PstI cleavage to 25% (T.C. Glenn et al., Biotechniques. 17, 1086-1090, 1994). PstI will not cut AGCTGCAG when methylated by AluI methyltransferase.
For methylation sensitivity refer to product specifications.