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Home | Molecular Biology | Restriction & Modifying Enzymes | Restriction Enzymes | Conventional

PsuI (BstYI)

5'...RG A T C Y...3'
3'...Y C T A GR...5'

Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available B buffer for 100% activity Optimal incubation at 37°C Thermal inactivation at 80°C in 20 min Recombinant enzyme LO certified

The PsuI (BstYI) restriction enzyme recognizes R^GATCY sites and cuts best at 37°C in B buffer (Isoschizomers: BstX2I, BstYI, MflI, XhoII)
  
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Lambda DNA digested with PsuI (BstYI)

Lambda DNA digested with PsuI (BstYI), 0.7% agarose, 21 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Compatible EndsBamHI, BclI, Bsp143I, MboI, BglII.
Conditions for 100% Activity
  • 1X Buffer B: 10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, and 0.1 mg/mL BSA
  • Incubate at 37°C
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
NoteHigh glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity.
Storage BufferPsuI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

New sites generated by ligation

Newly generated recognition sites resulting from ligation of protruding compatible DNA ends

Recognition Sequence Second Enzyme Enzymes recognizing newly generated recognition sequence
A^GATCY
  • BamHI/FastDigest BamHI (G^GATCC)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • PsuI (BstYI)/FastDigest PsuI
  • BclI/FastDigest BclI (T^GATCA)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I) (^GATC)
  • MboI/FastDigest MboI (^GATC)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • BglII/FastDigest BglII (A^GATCT)
  • BglII/FastDigest BglII
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • PsuI (BstYI)/FastDigest PsuI
G^GATCY
  • BamHI/FastDigest BamHI (G^GATCC)
  • BamHI/FastDigest BamHI
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • BspLI (NlaIV)/FastDigest NlaIV (BspLI)
  • BspPI (AlwI)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • PsuI (BstYI)/FastDigest PsuI
  • BclI/FastDigest BclI (T^GATCA)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I) (^GATC)
  • MboI/FastDigest MboI (^GATC)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • BspPI (AlwI)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • BglII/FastDigest BglII (A^GATCT)
  • Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)
  • BspPI (AlwI)
  • DpnI/FastDigest DpnI
  • MboI/FastDigest MboI
  • PsuI (BstYI)/FastDigest PsuI

Newly generated recognition sites resulting from fill-in of 5'-overhang and self-ligation

Recognition Sequence Newly generated sequence after reaction Enzymes that cleave the newly generated sequence
R^GATCYRGATCGATCY
  • [Bsp143I (Sau3AI)/FastDigest Sau3AI (Bsp143I)]
  • Bsu15I (ClaI)/FastDigest ClaI (Bsu15I)
  • [DpnI/FastDigest DpnI]
  • [MboI/FastDigest MboI]
  • TaqI/FastDigest TaqI