Previously added items:
5'...A G T↓A C T...3'3'...T C A↑T G A...5'
Lambda DNA digested with ScaI, 0.7% agarose, 5 cleavage sites
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Conditions of low salt, high enzyme concentration, glycerol concentration > 5% or pH > 8.0 may result in star activity. Supercoiled plasmids may require up to 20-fold more ScaI for complete digestion than linear DNA.
For methylation sensitivity refer to product specifications.