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SduI (Bsp1286I)

5'...G D G C H↓C...3'
3'...C↑H C G D G...5'
Available as a FastDigest enzyme for rapid DNA digestion

FastDigest enzyme available Unique buffer for 100% activity Optimal incubation at 37°C Thermal inactivation at 65°C in 20 min LO certified

The SduI (Bsp1286I) restriction enzyme recognizes GDGCH^C sites and cuts best at 37°C in its own unique buffer (Isoschizomers: Bsp1286I, MhlI)
  
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Lambda DNA digested with SduI (Bsp1286I)

Lambda DNA digested with SduI (Bsp1286I), 0.7% agarose, 38 cleavage sites

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

For methylation sensitivity refer to product specifications.

  
Storage Condition-20 C
HazardousNo
Compatible EndsAlw21I, ApaI, BseSI, Eco24I, Mph1103I, PstI, SacI, SdaI.
Conditions for 100% Activity
  • 1X Buffer SduI: 10 mM Tris-HCl (pH 7.2 at 37°C), 3 mM MgCl2, 150 mM NaCl, and 0.1 mg/mL BSA
  • Incubate at 37°C
Double DigestionPerform double digestion using DoubleDigest.
IsoschizomersSearch for commercial isoschizomers using REsearch.
NoteLow salt, high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme may result in star activity.
Storage BufferSduI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/mL BSA, and 50% (v/v) glycerol.

Reaction conditions

Recommended buffer for 100% activity Optimal temperature Enzyme activity in Thermo Scientific buffers, % Tango buffer for double digestion
B (blue)
1X		 G (green)
1X		 O (orange)
1X		 R (red)
1X		 Tango
(yellow)
1X / 2X
SduI Buffer (Unique) 37°C NR 50-100† 0-20 0-20 NR NR NR

† star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Methylation Effects

Methylation type Sequence Cleavage effect
Dcm (CCWGG) 5'...Cm5CW GGGCCm5CW GG...3'
3'...G GWm5CCCGG GWm5CC...5'		 No effect
CpG 5'...m5C GDGCHm5C G...3'
3'... Gm5CHCGD Gm5C...5'		 No effect

Cleavage efficiency close to the termini of PCR fragments

bp from the recognition site to fragment end
1 2 3 4 5
50-100

Number of recognition sites in DNA molecules

Lambda ΦX174 M13mp18/19 pBR322 puc18/19 pUC57
38 3 5 10 5 6
pTZ19R/U pTZ57R pBluescriptIIKS(±) pBluescriptIISK(±) pACYC177 pACYC184
5 6 6 6 4 8

New sites generated by ligation

Newly generated recognition sites resulting from ligation of protruding compatible DNA ends

Recognition Sequence Second Enzyme Enzymes recognizing newly generated recognition sequence
GAGCA^C
  • Alw21I (BsiHKAI)/FastDigest Alw21I* (GAGCA^C)
  • Alw21I (BsiHKAI)/FastDigest Alw21I
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GAGCC^C
  • Eco24I (BanII)* (GAGCC^C)
  • CviJI
  • Eco24I (BanII)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GAGCT^C
  • Alw21I (BsiHKAI)/FastDigest Alw21I* (GAGCT^C)
  • Eco24I (BanII)* (GAGCT^C)
  • SacI/FastDigest SacI (GAGCT^C)
  • AluI/FastDigest AluI
  • Alw21I (BsiHKAI)/FastDigest Alw21I
  • CviJI
  • Ecl136II (EcoICRI)/FastDigest Ecl136II
  • Eco24I (BanII)
  • SacI/FastDigest SacI
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
  • SetI
  • SetI* (AGCT)
  • AluI/FastDigest AluI
  • CviJI
  • SetI
GGGCA^C
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)* (GGGCA^C)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GGGCC^C
  • ApaI/FastDigest ApaI (GGGCC^C)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)* (GGGCC^C)
  • Eco24I (BanII)* (GGGCC^C)
  • ApaI/FastDigest ApaI
  • BmgT120I
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • Bsp120I (PspOMI)/FastDigest Bsp120I
  • BspLI (NlaIV)/FastDigest NlaIV (BspLI)
  • BsuRI (HaeIII)/FastDigest HaeIII (BsuRI)
  • Cfr13I (Sau96I)/FastDigest Sau96I (Cfr13I)
  • CviJI
  • Eco24I (BanII)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GGGCT^C
  • Eco24I (BanII)* (GGGCT^C)
  • CviJI
  • Eco24I (BanII)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GTGCA^C
  • Alw21I (BsiHKAI)/FastDigest Alw21I* (GTGCA^C)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)* (GTGCA^C)
  • Alw21I (BsiHKAI)/FastDigest Alw21I
  • Alw44I (ApaLI)/FastDigest ApaLI (Alw44I)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • Hpy8I (MjaIV)/FastDigest Hpy8I
  • HpyCH4V
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
  • Mph1103I (NsiI)/FastDigest NsiI (Mph1103I) (ATGCA^T)
  • HpyCH4V
  • PstI/FastDigest PstI (CTGCA^G)
  • SdaI (SbfI)/FastDigest SbfI (SdaI) (CCTGCA^GG)
  • BsgI
  • HpyCH4V
GTGCC^C
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)* (GTGCC^C)
  • BseSI (Bme1580I)/FastDigest Bme1580I (BseSI)
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)
GTGCT^C
  • Alw21I (BsiHKAI)/FastDigest Alw21I* (GTGCT^C)
  • Alw21I (BsiHKAI)/FastDigest Alw21I
  • SduI (Bsp1286I)/FastDigest Bsp1286I (SduI)