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Thermo Scientific M-MuLV Reverse Transcriptase (RT) is an RNA- and DNA-dependent DNA polymerase. It can use either RNA or DNA to prime DNA synthesis. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids (see References 1, 2).
M-MuLV RT has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase.
I. M. Verma, Reverse transcriptase, The Enzymes (P.D. Boyer, ed, Academic Press Inc., vol. 14, 1981) pp. 87-103.
G. F. Gerard, J. M. D’Alessio, Methods in Molecular Biology (Humana Press, N.J. Totowa, vol. 16, 1993) pp. 73-93.
J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2001).