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RevertAid First Strand cDNA Synthesis Kit

Store at -20°C

A complete system for efficient synthesis of first strand cDNA from RNA templates.
  
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Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5’-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Highlights

  • Full-length first strand cDNA up to 13 kb
  • Optimum reaction temperature 42°C
  • Complete kit – all the components for the RT reaction are included

Applications

  • First strand cDNA synthesis for RT-PCR and RT-qPCR
  • Construction of full length cDNA libraries
  • Antisense RNA synthesis

Includes

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.

  
Storage Condition-20 C
HazardousNo
Quality ControlThe kit is functionally tested in RT-PCR using 100 fg control GAPDH RNA and GAPDH control primers generated a 496 bp product visible on agarose gel after ethidium bromide staining.
PCR-amplified regions from mouse dystrophin cDNA - gel image

Full-length cDNA synthesis using the RevertAid First Strand cDNA Synthesis Kit

PCR-amplified regions from mouse dystrophin cDNA - gel image

1 µg of total mouse heart RNA was used in a reverse transcription reaction using oligo(dT)18 primer. The synthesized cDNA was used as a template in subsequent PCR reactions with Taq DNA Polymerase. The ability to amplify the terminal 2024 bp fragment indicates that the entire 13.8 kb RNA sequence was reverse-transcribed.