Previously added items:
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain (see References 1, 2). RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).
Also available: RevertAid H Minus First Strand cDNA Synthesis Kit
I. M. Verma, Reverse transcriptase, The Enzymes, Boyer, P.D., ed (Academic Press Inc., Philadelphia, PA, 1981), vol. 14, pp. 87-103.
G. F. Gerard, J. M. D’Alessio, Methods in Molecular Biology (Humana Press, Totowa, NJ,1993), vol. 16, pp. 73-93.
J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, ed. 3, 2001). [third edition]
A. Schmidt et al., UPS1 and UPS2 from Arabidopsis Mediate High Affinity Transport of Uracil and 5-Fluorouracil. J. Biol. Chem. 279, 44817-44824 (2004).
K. G. Papavinasasundaram et al., Deletion of the Mycobacterium tuberculosis pknH Gene Confers a Higher Bacillary Load during the Chronic Phase of Infection in BALB/c Mice. J. Bacteriol. 187, 5751-5760 (2005).
R. Turk et al., Gene expression variation between mouse inbred strains. BMC Genomics. 5, 57 (2004).
F. L. Pinto, P. Lindblad, A guide for in-house design of template-switch-based 5’rapid amplification of cDNA ends systems. Analytical Biochemistry. 397, 227-232 (2010).