siGLO RNAi Controls

siGLO RNAi Controls

Fluorescent controls are useful for visual indication of delivery, and when combined with functional knockdown assessment, provide a useful tool for optimization.

siGLO Positive controls will effectively silence the indicated gene, and result in punctate cytoplasmic fluorescence. All are labeled with DY-547 (Cy3 analog).

siGLO RISC-Free Control is a DY-547-labeled negative control that can also be co-transfected with functional siRNAs.

siGLO Transfection Indicators are unique non-RISC engaging molecules that localize to the nucleus, providing a distinct visual indication of transfection success. They are available with either DY-547 (Red) or 6-FAM (Green).

siGLO Positive Control Reagents Species Catalog #
siGLO Cyclophilin B Control siRNA H, M, R D-001610-01
siGLO Lamin A/C siRNA H, M, R D-001620-01
siGLO Lamin A/C Control siRNA H D-001620-02
siGLO Lamin A/C Control siRNA M D-001620-03
siGLO Lamin A/C Control siRNA R D-001620-04
siGLO Negative Control Reagents Species Catalog #
siGLO RISC-Free Control H, M, R D-001600-01
siGLO Transfection Indicators Species Catalog #
siGLO Green Transfection Indicator H, M, R D-001630-01
siGLO Red Transfection Indicator H, M, R D-001630-02

H=human, M=mouse, R=rat