Fluorescent controls are useful for visual indication of delivery, and when combined with functional knockdown assessment, provide a useful tool for optimization.
siGLO Positive controls will effectively silence the indicated gene, and result in punctate cytoplasmic fluorescence. All are labeled with DY-547 (Cy3 analog).
siGLO RISC-Free Control is a DY-547-labeled negative control that can also be co-transfected with functional siRNAs.
siGLO Transfection Indicators are unique non-RISC engaging molecules that localize to the nucleus, providing a distinct visual indication of transfection success. They are available with either DY-547 (Red) or 6-FAM (Green).
| siGLO Positive Control Reagents |
Species |
Catalog # |
| siGLO Cyclophilin B Control siRNA |
H, M, R |
D-001610-01 |
| siGLO Lamin A/C siRNA |
H, M, R |
D-001620-01 |
| siGLO Lamin A/C Control siRNA |
H |
D-001620-02 |
| siGLO Lamin A/C Control siRNA |
M |
D-001620-03 |
| siGLO Lamin A/C Control siRNA |
R |
D-001620-04 |
| siGLO Negative Control Reagents |
Species |
Catalog # |
| siGLO RISC-Free Control |
H, M, R |
D-001600-01 |
| siGLO Transfection Indicators |
Species |
Catalog # |
| siGLO Green Transfection Indicator |
H, M, R |
D-001630-01 |
| siGLO Red Transfection Indicator |
H, M, R |
D-001630-02 |
H=human, M=mouse, R=rat