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SMARTchoice Lentiviral shRNA Control Particles


SMARTchoice positive and negative controls are offered with your choice of seven different promoters (human and mouse CMV, human and mouse EF1 alpha, CAG, PGK, and UBC) and two flourescent reporters (TurboGFP or TurboRFP). Order matched controls for your tailored SMARTvector 2.0 gene-specific constructs.
  
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Thermo Scientific SMARTchoice shRNA Control Particles are SMARTvector 2.0 positive and negative controls which include your choice amongst seven different promoters and two fluorescent reporters. If the optimal promoter for your specific cells of interest has yet to be identified, we recommend utilizing the SMARTchoice shRNA Promoter Selection Plate to evaluate multiple promoters in a single experiment.

SMARTvector 2.0 Positive Controls

Highlights

  • SMARTvector 2.0 positive control constructs provied as packaged lentiviral particles
  • Consistent, potent silencing of non-essential, abundantly expressed housekeeping genes in human (see Figure 1), mouse and rat cells

Applications

  • Confirm transduction efficiency via TurboGFP or TurboRFP expression
  • Evaluate specific and potent silencing of housekeeping gene

SMARTvector 2.0 Negative Controls

Highlights

  • SMARTvector 2.0 negative control constructs provied as packaged lentiviral particles
  • Validated to have minimal targeting of any known genes in human, mouse and rat genomes

Applications

  • Confirm silencing specificity of gene-targeting SMARTvector 2.0 Lentiviral shRNA Particles
  • Distinguish sequence-specific silencing from non-specific effects
  
Shipping ConditionDry Ice
Storage Condition-80 C
Shelf Life12 Months
HazardousNo
SMARTvector 2.0 Lentiviral shRNA Control Particles Give Consistent Results Across Cell Lines

SMARTvector 2.0 Lentiviral shRNA Control Particles Give Consistent Results Across Cell Lines

SMARTvector 2.0 Lentiviral shRNA Control Particles Give Consistent Results Across Cell Lines

Figure 1. SMARTvector 2.0 Lentiviral shRNA Controls: Non-Targeting Control and GAPD. K562, HUVEC, and SH-SY5Y cells were transduced at three MOIs and assessed for mRNA knockdown 72 hours post-transduction using a branched DNA assay (Panomics, Inc.)