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The RNAi consortium (TRC) is a collaborative effort based at the Broad Institute of MIT and Harvard, and includes six MIT- and Harvard-associated research institutions and five international life sciences organizations. We have partnered with the TRC to make the shRNA libraries available to researchers worldwide. Available TRC shRNA controls include the pLKO.1 empty vector as a negative control and an eGFP shRNA as a positive control.
The pLKO.1 controls can be packaged into virus particles for use in transduction experiments. Specifically, the replication-incompetent viral particles can be efficiently produced using lentiviral packaging plasmids co-transfected in 293T packaging cells. The Trans-Lentiviral packaging system, which offers maximum biosafety and high titers, is recommended.
Further information is available at http://www.broad.mit.edu/genome_bio/trc/rnai.html
In scientific publications, the libraries should be referred to as TRC-Hs1.0 (Human) and TRC-Mm1.0 (Mouse). Individual clones are uniquely identified by their TRC ID number (eg. TRCN0000014783).