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Thermo Scientific TurboFect in vivo Transfection Reagent is highly effective for DNA plasmid delivery in vivo, while not inducing an inflammatory response. TurboFect in vivo has been assessed for plasmid DNA delivery in both mice and rats and is suitable for DNA administration via various routes, including intravenous, intraperitoneal, and intratumoral injection. The amount of DNA and suggested injection volume will depend on the experimental animal and the route of administration as well as the targeted tissue or organ and the expression vector.
TurboFect in vivo is a sterile solution of a cationic polymer in water. The polymer forms compact, stable, positively charged complexes with DNA. These complexes protect DNA from degradation and facilitate gene delivery in vivo.
Figure 1. | No inflamatory response after DNA delivery using TurboFect in vivo Transfection Reagent.50 μg of plasmid DNA was delivered into female BALB/c mice via intravenous injection using TurboFect in vivo Transfection Reagent. The concentration of proinflamatory cytokine TNFα was determined by ELISA in blood serum samples taken at the indicated time points. LPS – lipopolysaccharides.
Figure 2. | Comparison of TurboFect in vivo Transfection Reagent with other in vivo transfection reagents.50 μg of plasmid DNA coding for firefly luciferase was complexed with in vivo transfection reagents and delivered into female BALB/c mice via intravenous injection. Transfections were performed according to manufacturer's recommendations. Firefly luciferase expression was determined in various organs 24 h after plasmid DNA delivery.