TurboFect Transfection Reagent


TurboFect Transfection Reagent
A novel formulation for efficient in vitro delivery of plasmid DNA to cultured cells with low cytotoxicity.
  
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Manufactured by Thermo Scientific. Sold and distributed by both Thermo Scientific and GE Healthcare.

TurboFect Transfection Reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

Highlights

  • High transfection efficiency of plasmid DNA into a wide variety of cell types
    • Adherent and suspension cells
    • Differentiated and undifferentiated cells
  • Excellent transfection efficiency in the presence or absence of serum 
  • Ready-to-use reagent – no need to reconstitute, dilute, or manipulate
  • Simple protocol
EGFP expression in cell lines transfected with TurboFect reagent

Figure 1. EGFP expression in cell lines transfected with TurboFect in vitro Transfection Reagent.
(A) – primary mouse dendritic cells derived from bone marrow
(B) – HeLa cells

Review our experimental considerations to improve your plasmid transfection results.
  
HazardousNo
Shelf Life24 Months
Shipping ConditionRefrigerated Gel Packs
Storage Condition4 C
Comparison of TurboFect with tranfection reagents from other vendors

Comparison of TurboFect with tranfection reagents from other vendors

Comparison of TurboFect with tranfection reagents from other vendors

Figure 2. Comparison of TurboFect Transfection Reagent with tranfection reagents from other vendors.

HeLa cells were transfected with plasmid DNA encoding eGFP. Transfections were performed according to manufacturers' recommendations and analysed by flow cytometry.


High transfection efficiency of a wide variety of cell types

High transfection efficiency of a wide variety of cell types

High transfection efficiency of a wide variety of cell types

Figure 3.


References

Citations