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Figure 1. EGFP expression in cell lines transfected with TurboFect in vitro Transfection Reagent.(A) – primary mouse dendritic cells derived from bone marrow(B) – HeLa cells
Comparison of TurboFect Transfection Reagent with transfection reagents from other vendors. HeLa cells were transfected with plasmid DNA encoding eGFP. Transfections were performed according to manufacturers' recommendations and analysed by flow cytometry.
Tables list cell types that have been evaluated with TurboFect transfection reagent for efficiency and cytotoxicity. Results stated are for transient (48 hour) transfection. 1 μg DNA plasmid expressing GFP : 1.8-2.2 μL TurboFect transfection reagent.*Transfection efficiency measured by percentage of GFP positive cells in the population of live cells by flow cytometry.**Cell viability measured by % of live/dead cells from total population.