TurboFect Transfection Reagent


TurboFect Transfection Reagent
A novel formulation for efficient in vitro delivery of plasmid DNA to cultured cells with low cytotoxicity.
  
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TurboFect Transfection Reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

Highlights

  • High transfection efficiency of plasmid DNA into a wide variety of cell types
    • Adherent and suspension cells
    • Differentiated and undifferentiated cells
  • Excellent transfection efficiency in the presence or absence of serum 
  • Ready-to-use reagent – no need to reconstitute, dilute, or manipulate
  • Simple protocol
EGFP expression in cell lines transfected with TurboFect reagent

Figure 1. EGFP expression in cell lines transfected with TurboFect in vitro Transfection Reagent.
(A) – primary mouse dendritic cells derived from bone marrow
(B) – HeLa cells

Review our experimental considerations to improve your plasmid transfection results.
  
HazardousNo
Shelf Life24 Months
Shipping ConditionRefrigerated Gel Packs
Storage Condition4 C
Comparison of TurboFect Transfection Reagent with transfection reagents from other vendors.

Comparison of TurboFect Transfection Reagent with transfection reagents from other vendors.

Comparison of TurboFect Transfection Reagent with transfection reagents from other vendors.

Comparison of TurboFect Transfection Reagent with transfection reagents from other vendors.
HeLa cells were transfected with plasmid DNA encoding eGFP. Transfections were performed according to manufacturers' recommendations and analysed by flow cytometry.


High transfection efficiency in a wide variety of cell types

High transfection efficiency in a wide variety of cell types

High transfection efficiency in a wide variety of cell types

Immortalized Cell Cultures
Cell line Origin % Transfected cells* % Viability**
Cos-7 African green monkey kidney cells 98 90
HeLa Human cervix adenocarcinoma cells 97 95
CHO Chinese hamster ovary cells 89 92
HEK293 Human embryonic kidney cells 94 80
B50 Rat nervous tissue neuronal cells 60 87
Calu1 Human lung epidermoid carcinoma cells 70 90
RAW264 Mouse leukaemic monocyte-macrophage cells 60 93
WEHI Mouse B cell lymphoma cells 75 87
MDCK Madin Darby Canine Kidney cells 92 94
Raji Human Burkitt's lymphoma cells 0.2 87
COLO Human colon adenocarcinoma cells 20 90
Jurkat Human leukaemic T cells 5 77
Sp2/Ag14 Mouse myeloma cells 22 97
HeLa S3 Human cervix carcinoma cells 32 85
Hep2C Human larynx carcinoma cells 90 88
L929 Mouse connective tissue fibroblasts 60 91
NIH3T3 Mouse embryo fibroblasts 97 89
HepG2 Human hepatoma cells 55
BHK 21 Baby hamster kidney cells 84
Primary Cell Cultures
Cell line Origin % Transfected cells* % Viability**
Dendritic cells Mouse bone marrow 5 76
Macrophages Mouse bone marrow 3 48
HLF Human lung fibroblasts 91 88

Tables list cell types that have been evaluated with TurboFect transfection reagent for efficiency and cytotoxicity. Results stated are for transient (48 hour) transfection.

1 μg DNA plasmid expressing GFP : 1.8-2.2 μL TurboFect transfection reagent.*Transfection efficiency measured by percentage of GFP positive cells in the population of live cells by flow cytometry.**Cell viability measured by % of live/dead cells from total population.


References

Citations