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Thermo Scientific TurboFect Transfection Reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.
Figure 1. EGFP expression in cell lines transfected with TurboFect in vitro Transfection Reagent.(A) – primary mouse dendritic cells derived from bone marrow(B) – HeLa cells
Review our experimental considerations to improve your plasmid transfection results.
Figure 2. Comparison of TurboFect Transfection Reagent with tranfection reagents from other vendors.
HeLa cells were transfected with plasmid DNA encoding eGFP. Transfections were performed according to manufacturers' recommendations and analysed by flow cytometry.