• Web Tools

    Web Tools
    Online tools for analyzing primers, setting up reactions
    and performing biochemical conversions & calculations.

    Primer design and analysis Tools for planning restriction digestion

    Analyzes the Tm, MW and extinction coefficient of a primer, and gives dilution instructions.


    Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation etc.

    Performs double digestion with Thermo Scientific restriction enzymes.


    Finds restriction enzymes either by name or recognition sequence.

    Reaction setup calculators Biochemical conversions

    PCR reaction setup with Thermo Scientific Phusion® DNA Polymerases.


    qPCR reaction setup with Thermo Scientific DyNAmo™ qPCR Kits.


    cDNA synthesis reaction setup with Thermo Scientific DyNAmo™ cDNA Synthesis Kit.

    Calculates DNA and RNA concentrations based on measured OD260 values.


    Analyzes qPCR amplification efficiency based on the slope of the standard curve.


    Calculates copy numbers for DNA originating from different organisms.

    PCR fidelity RNAi Screening

    Compare the error rate of Thermo Scientific Phusion® High-Fidelity DNA Polymerase with other DNA polymerases.

    siRNA and transfection reagent calculators

    Estimate quantities of siRNA/microRNA and DharmaFECT needed for siRNA screens

    REviewer siRNA Seed Locator
    REviewer: Fidelity calculator for Phusion® DNA Polymerases

    Provides an integrated set of free tools for DNA sequence analysis, plasmid map creation, rich sequence viewing and various biochemical calculations.

    This web-based tool is available to the scientific community in consideration of the on-going efforts to develop intelligent and effective tools for selecting viable siRNA candidates and advancing the rational design of such.

    Modification Percentage Calculation  
    5-hmC Percentage Calculator

    Calculates 5-hmC percentage using the Thermo Scientific EpiJET 5-hmC Analysis Kit(#K1481)